DNA immunization using a non-viral promoter

DNA immunization and viral load in vaccinated trout following experimental infection with infectious pancreatic necrosis virus (IPNV)

Viral hepatitis prevention by immunization.

OBJECTIVE To present an updated review and criticism of viral hepatitis A and B prevention by immunization. SOURCES OF DATA Review of medical articles obtained from the MEDLINE database. The most recent and representative articles on the subject (2000-2006) were selected. The Centers for Disease Control and Prevention (CDC), American Academy of Pediatrics (AAP), Brazilian Society of Pediatric...

A novel formulation of Mtb72F DNA vaccine for immunization against tuberculosis

Objective(s): Mycobacterium tuberculosis (M. tuberculosis), an intracellular pathogen, causes 1.5 million deaths globally. Bacilli Calmette-Guérin (BCG) is commonly administered to protect people against M. tuberculosis infection; however, there are some obstacles with this first-generation vaccine. DNA vaccines, the third generation vaccines, can induce cellular immun...

mRNA delivery using non-viral PCL nanoparticles.

Messenger RNA (mRNA) provides a promising alternative to plasmid DNA as a genetic material for delivery in non-viral gene therapy strategies. However, it is difficult to introduce mRNA in vivo mainly because of the instability of mRNA under physiological conditions. Here, mRNA-protamine complex encapsulated poly(ε-caprolactone) (PCL) nanoparticles (NPs) are proposed for the intracellular delive...

The Intracellular Fate of Non-viral Dna Carriers

Rapid progress in molecular biology and genetic engineering techniques has made the induction of cell behavior modifications by altering genetic material possible. This allows us to think about the medical application of gene therapy as an alternative to classical pharmacology and as a means of introducing a new approach to treating hereditary diseases. The major obstacle that prevents the appl...